FIG 5.

Effect of L-161,982 on preformed mono- and dual-species biofilms. The effect of L-161,982 on preformed mature biofilms was tested. Mono- and dual-species C. albicans (C.a) and/or S. aureus (S.a) biofilms were grown on 96-well plates for 24 h before being treated with the indicated concentration of L-161,982 for a further 24 h. After treatment, the metabolic activity of the biofilms was quantified by the XTT assay (A), and then the biofilms were disrupted to determine the number of live CFU (B). The results are cumulative results from three independent experiments with duplicate samples. Statistical analysis was performed using one-way ANOVA with the Tukey post hoc test for the XTT assay. The Mann-Whitney U test was used to analyze the CFU data. Significance is indicated as follows: ns, not significant; *, P < 0.05; **, P < 0.01. Error bars represent standard deviations.