The combinations of vitamin C and TB drugs sterilize M. tuberculosis cultures. M. tuberculosis H37Rv was grown to mid-log (OD600, 0.6 to 0.8), as described in Materials and Methods, diluted 1:100, and treated for 4 weeks with vitamin C (vitC, 1 mM), a combination of INH (7 μM) and RIF (1.2 μM), or the combination INH (7 μM)/RIF (1 μM)/vitC (1 mM) (A); or vitamin C (vitC, 1 mM), a combination of ofloxacin (OF, 14 μM), kanamycin (Km, 41 μM), and ethionamide (ETH, 150 μM), or the combination OF (14 μM)-Km (41 μM)-ETH (150 μM)-vitC (1 mM) (B). INH, RIF, OF, Km, and ETH were used at concentrations equivalent to 10 times their MIC. Aliquots were taken at indicated times and plated to determine the CFU. The plates were incubated at 37°C for 4 to 6 weeks. The mean and standard deviation are plotted (n = 3).