FIG 5.

fhp expression increases over time during biofilm development. fhp mRNA levels were quantified by qRT-PCR analysis and compared at different time points in biofilms grown at 37°C with fast agitation (A) or compared between 6-h biofilms grown at 37°C with fast agitation and 20-h biofilms grown at room temperature with slower agitation, a system that allows continuous increase in biofilm biomass over 20 h (B). (C) Time course analysis of fhp mRNA levels in the 20-h biofilm system. The error bars indicate standard errors (n = 3). The asterisks indicate statistically significant differences between samples (**, P < 0.01).