FIG 6.

HSP90 is a Rim-dependent gene. (A) Normalized gene counts obtained by RNA-Seq at both acidic and alkaline pHs for the HSP90, CMP1, and MKC1 genes in the SC5413 reference strain, the rim101Δ disruption strain (DAY25), and the RIM101-overexpressing strain (CGY1). (B) HSP90 expression levels obtained by RT-qPCR at acidic and alkaline pHs for the rim101Δ disruption strain DAY25, the complemented strain DAY5SL, and the control strain DAY185, normalized to the ACT1 level. (C) Colony formation assays in the presence of the pharmacological Hsp90 inhibitor geldanamycin. Cells were plated in 10-fold dilutions in the presence of geldanamycin (10 μM) in SC medium buffered at pH 7.2. The rim mutants were hypersensitive to pharmacological inhibition of Hsp90.