FIG 2.

Ada2 is required for thermotolerance and growth. (A) The growth of ada2 mutants was temperature sensitive, and the osmotic stabilizer sorbitol partially rescued the phenotype at 40°C. Cells were grown overnight in YPD at 37°C and washed twice with dH₂O, 5-fold serially diluted, and spotted onto solid YPD with or without 1 M sorbitol. The cultures were incubated at the indicated temperature for 48 h. (B) Growth kinetics of C. glabrata strains at 25°C, 37°C, or 40°C. Cells were grown overnight in liquid YPD at 37°C, washed twice with dH₂O, diluted to an OD₆₀₀ of 0.1 unit/ml in fresh liquid YPD, and incubated at the indicated temperature with shaking at 150 rpm. The OD₆₀₀ of the cultures was measured at 0, 3, 6, 9, 12, 15, 18, 24, 48, and 72 h. The results were plotted using Prism (v5.03) software, and the error bars represent standard deviations from triplicate technical experiments.