FIG 4.

Inactivation of ADA2 enhances agar invasion. (A) C. glabrata strains were grown overnight in YPD at 37°C, washed twice with dH₂O, and diluted to an OD₆₀₀ of 1 unit/ml. Then, 3 μl of cells was spotted onto solid YPD and incubated at 37°C for 10 days. Colonies were photographed before and after washing with dH₂O. (B) The colonies from the assay whose results are shown in panel A were washed, excised, and microscopically observed from the top and side. Arrows, the agar surface.