Figure 12.

Proposed working hypothesis/model for future validation and establishment of a possible co-ordination and participation of relish (REL)/nitric oxide (NO) synthase controlling antimicrobial peptides response. To establish possible flow of signal relationship, we interpreted the cross tissue experimental data as below: (i) Endogenous bacterial feeding: demonstrating NO dominantly regulate flow signal from midgut (MG)-to-FB and/or MG-to-hemocyte (HC) (Table S2 in Supplementary Material). (ii) Exogenous bacterial injection: demonstrating REL dominantly regulate flow signal from FB-to-MG; while NO regulate HC-to-MG (Table S2 in Supplementary Material). A synergistic relationship of REL/NO between FB and HC was established using combined data from individual as well mixed bacterial challenge experiments.