Figure 3.

Real time (RT)-PCR expression analysis of antimicrobial peptides (AMPs) during the development of mosquito (A); tissue-specific relative expression analysis (B,C); silencing validation of cecropin family genes (D–F); effect of AMPs silencing in the FB and MG (G–I). The relative expression was monitored using RT-PCR assays. Three independent biological replicates were considered for statistical analysis viz. *p < 0.05; **p < 0.005; ***p < 0.0005, using Student’s t-test; NS, not significant. Abbreviations: FB, fat body; MG, midgut; SGs, salivary glands.