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. 2018 Feb 20;9:317. doi: 10.3389/fimmu.2018.00317

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Copyright © 2018 Lakhrif, Moreau, Hérault, Di-Tommaso, Juste, Moiré, Dimier-Poisson, Mévélec and Aubrey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specific antibody response in mice immunized with untargeted (SAG1t) or targeted (SA2) proteins. Detection of specific anti-SAG1 IgG antibodies (A) and IgG subclasses (B) in sera of mice (12/group) primed and boosted twice with SAG1t, SA2, or phosphate-buffered saline formulated with Poly I:C by combined routes. Serum samples collected after the last boost were tested by ELISA using SAG1t protein as the coating antigen. Results are expressed as the mean ± SEM (n = 12) of log2 titers and represent one of two independent experiments. ***P < 0.001. (C) Immunofluorescence assay of Toxoplasma gondii tachyzoites with sera of mice immunized with SAG1t or SA2 proteins. Tachyzoites were labeled with anti-Mouse IgG-TRITC antibody (1:200) after incubation with sera. Serum from non-infected and infected mice were used for negative and positive controls, respectively. (D) Western blot analysis of the IgA antibody response in nasal washes. IgA specific for SAG1 protein were analyzed 1 week after the last boost, in nasal washes from four mice of each group (control, SAG1t, and SA2).