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. 2018 Feb 20;9:317. doi: 10.3389/fimmu.2018.00317

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Copyright © 2018 Lakhrif, Moreau, Hérault, Di-Tommaso, Juste, Moiré, Dimier-Poisson, Mévélec and Aubrey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cellular response after vaccination with SAG1t or SA2 by the combined routes. CBA/J mice were primed and boosted twice by combined intranasal and subcutaneous routes with SAG1t, SA2, or phosphate-buffered saline formulated with polyinosinique-polycytidylique acid adjuvant. 7 days after the last immunization, mesenteric lymph node cells (A) and splenocytes (B) were collected from four mice in each group and restimulated with TE. Supernatants were collected after 24 (IL-2) or 72 h (IFN-γ, IL-10, and IL-13) for cytokines assay. Results are expressed as the mean ± SEM and represent one of two independent experiments. **P < 0.01.