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. 2018 Feb 20;9:255. doi: 10.3389/fmicb.2018.00255

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Copyright © 2018 Bojer, Lindemose, Vestergaard and Ingmer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Persister reducing activity does not require induction of the agr response. Persister frequencies of S. aureus strains RN6607 (agr group II) (A) and MOZ53 (agr group III) (B) in the presence of 25% stationary phase endogenous supernatants or supernatant obtained from strain Newman (agr group I). (C) Effect of the wild type strain Newman stationary phase supernatant (25%) on persister cells isolated from strain NewmanΔagr. Persister cells were isolated using 20x MIC of ciprofloxacin and all supernatant treatments were compared to the un-supplemented control (TSB). The data represent the mean persister frequencies ± SD calculated from three biological replicates (^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001).