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. 2018 Feb 20;9:255. doi: 10.3389/fmicb.2018.00255

FIGURE 7.

FIGURE 7

Phenol soluble modulins are strong determinants of S. aureus persister levels in stationary phase. (A) S. aureus strain NewmanΔagr stationary phase persister frequencies obtained when supplemented with 50% stationary phase supernatants from either strain LAC or LACΔpsmαβhld. Persister cells were selected using 100x MIC of ciprofloxacin and treatments were compared to the control added endogenous supernatant only. (B) Stationary phase persister frequencies of S. aureus strain Newman and the derived psmα mutant selected with 100x MIC ciprofloxacin added directly to overnight cultures. (C) Stationary phase persisters of strain Newman (WT) and NewmanΔagr (Δagr) when resuspended in 90% endogenous supernatant prior to challenge with 100x MIC ciprofloxacin in comparison to persister level obtained with NewmanΔagr in supernatant derived from WT. The data represent the mean persister frequencies ± SD calculated from three biological replicates (∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).