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. 2018 Feb 20;12:86. doi: 10.3389/fnins.2018.00086

Figure 2.

Figure 2

Altered solubility of SOD1 in SOD1 and C9orf72 motor neurons. (A) Representative immunocytochemistry images of Hoechst nuclear stain (blue), SOD1 (green), and Tuj1 (white) at 4 weeks of differentiation shows no significant difference in the number of neurons with SOD1+ puncta in ALS motor neurons compared to control (ns, p > 0.05 by one-way ANOVA with Tukey post-hoc test). C9orf72 and TDP-43 motor neurons exhibited fewer SOD1+ puncta per neuron compared to control (*p < 0.05 by one-way ANOVA with Tukey post-hoc test). n = 3, scale bar = 10 μm (full image) or 2 μm (expanded). (B) Western blot for SOD1 shows no significant difference in ALS lines compared to control lines. Data are normalized to REVERT total protein stain and expressed as a fold change over control (ns, p > 0.05 by one-way ANOVA with Tukey post-hoc test, n = 3–6). (C) Filter-trap assay shows a trend toward increased levels of insoluble SOD1 in SOD1 and C9orf72 motor neurons but not in iPSC colonies (ns, p > 0.05 by one-way ANOVA with Tukey post-hoc test). Data are expressed as fold change over controls. n = 3.