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. 2018 Feb 20;12:86. doi: 10.3389/fnins.2018.00086

Figure 3.

Figure 3

Alterations in TDP-43 solubility in TDP-43 motor neurons. (A) Representative images at 4 weeks of differentiation show no change in the number of neurons with cytoplasmic TDP-43+ puncta or the number of TDP-43+ puncta per neuron in ALS motor neuron cultures (ns, p > 0.05 by one-way ANOVA). n = 3, scale bar = 10 μm (full image) or 2 μm (expanded). (B) Western blot for TDP-43 shows no significant difference in protein levels in ALS motor neuron cultures. Data are normalized to REVERT total protein stain and expressed as a fold change over control (ns, p > 0.05 by one-way ANOVA, n = 3–5). (C) Filter-trap assay of TDP-43 shows a trend toward an increase in insoluble levels in TDP-43 motor neurons but not in iPSC colonies (ns, p > 0.05 by one-way ANOVA with Tukey post-hoc test). Data are expressed as fold change over controls, n = 3.