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. 2018 Feb 20;12:86. doi: 10.3389/fnins.2018.00086

Figure 4.

Figure 4

OPTN is insoluble in SOD1 and C9orf72 motor neurons. (A) Representative immunocytochemistry images of Hoechst nuclear stain (blue), OPTN (green), and Tuj1 (white) at 4 weeks of differentiation. No difference is seen in the number of neurons with OPTN+ puncta (ns, p > 0.05 by one-way ANOVA with Tukey post-hoc test). n = 3, scale bar = 10 μm (full image) or 2 μm (expanded). (B) Total protein levels of OPTN are unchanged by Western blot. Data are normalized to REVERT total protein and expressed as a fold change over control (ns, p > 0.05 by one-way ANOVA with Tukey post-hoc test). n = 3. (C) OPTN filter-trap assay shows a trend toward increased insoluble protein in SOD1 and C9orf72 motor neurons (ns, p > 0.05 by one-way ANOVA with Tukey post-hoc test). Insoluble OPTN was observed in the colonies from one C9orf72 iPSC line, but in no others. Data are expressed as fold change over controls, n = 3.