Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Nfe2l2 | NA | MGI:108420 | |
| Strain, strain background (M. musculus) | YWK | PMID: 8943040 | Nrf2-/-; YWK Strain | |
| Strain, strain background (M. musculus) | MYM | PMID: 9240432 | Nrf2-/-; MYM Strain | |
| Strain, strain background (M. musculus) | PER2::LUC | PMID: 14963227 | PER2::LUC | |
| Genetic reagent (M. musculus) | Per2:Luc | PMID: 23052244; 24699442; 18454201; 17482552 | Per2:Luc | |
| Cell line (M. musculus) | Wt MEF Per2:Luc | this paper | Wt MEF Per2:Luc | Immortalized Wt MEF cell line stably expressing a Per2:Luc reporter. Created by Thomas R. Sutter's Lab at the University of Memphis. |
| Cell line (M. musculus) | Nrf2-/- MEF Per2:Luc | this paper | Nrf2-/- MEF Per2:Luc | Immortalized Nrf2-/- MEF cell line stably expressing a Per2:Luc reporter. Created by Thomas R. Sutter's Lab at the University of Memphis. |
| Cell line (M. musculus) | Keap1-/- MEF Per2:Luc | this paper | Keap1-/- MEF Per2:Luc | Immortalized Keap1-/- MEF cell line stably expressing a Per2:Luc reporter. Created by Thomas R. Sutter's Lab at the University of Memphis. |
| Cell line (M. musculus) | MMH-D3 Per2:Luc | PMID: 24699442 | MMH-D3 Per2:Luc | |
| Antibody | anti-Nrf2 (rabbit polyclonal) | Santa Cruz | Santa Cruz SC-722 | (1:500) |
| Antibody | anti-Nqo1 (rabbit monoclonal) | Abcam | Abcam AB80588 | (1:10,000) |
| Antibody | anti-Nr1d1 (rabbit monoclonal) | Cell Signaling | Cell Signaling 13418 | (1:500) |
| Antibody | anti-Prdx-SO2/3 (rabbit polyclonal) | Thermo Fisher | Thermo LF-PA0004 | (1:1000) |
| Antibody | anti-Per2 (rabbit polyclonal) | ProteinTech | ProteinTech 20359 | (1:200) |
| Antibody | anti-Nrf2 (rabbit polyclonal) | Diagenode | Diagenode C15410242 | (2 ug) |
| Recombinant DNA reagent | shNrf2 (shRNA) | Sigma | Sigma NM_010902:TRCN0000012128 | |
| Recombinant DNA reagent | shNrf2 (shRNA) | this paper | shNrf2-1 | shNrf2 lentiviral vectorwas developed byAndrew Liu at theUniversity of Memphis. Target sequence is described in Supplementary file 4. |
| Recombinant DNA reagent | shNrf2-2 (shRNA) | this paper | shNrf2-2 | shNrf2 lentiviral vectorwas developed byAndrew Liu at theUniversity of Memphis. Target sequenceis described in Supplementary file 4. |
| Recombinant DNA reagent | shNrf2-3 (shRNA) | this paper | shNrf2-3 | shNrf2 lentiviral vectorwas developed byAndrew Liu at theUniversityof Memphis. Target sequenceis described in Supplementary file 4. |
| Recombinant DNA reagent | CMV-Nrf2 (Expression vector) | this paper | CMV-Nrf2 | CMV-driven Nrf2expression constructwas created inthe pLV7 lentiviral vector backbone usingGateway recombination. Primers to amplifyNrf2 cDNA and shuttlethe ORF into pLV7 are described in Supplementary file 3. |
| Recombinant DNA reagent | pLENTI-Nrf2 (Expression vector) | Origene | Origene MR226717L1 | |
| Recombinant DNA reagent | pGL3-P(Per1)-dLuc | PMID: 22692217 | Per1:Luc | |
| Recombinant DNA reagent | pGL3-3xEbox-P(SV40)-dLuc | PMID: 22692217 | 3xEbox:Luc | |
| Commercial assay or kit | Lenti-X p24 Rapid Titer Kit | Clontech | Clontech 632200 | |
| Chemical compound, drug | Hydrogen peroxide | Sigma | Sigma 216763 | |
| Chemical compound, drug | D3T | LKT Laboratories | LKT Laboratories D0010 | |
| Software, algorithm | LumiCycle Analysis | Actimetrics | Version 2.31 |