Author response image 8. FoxP2 expression in Area X is elevated for up to 30 days following AAV-FoxP2 injection.

Juvenile birds (~30d) were injected with our FoxP2 overexpressing construct, bilaterally into Area X. FoxP2 levels were examined 10 days (n=3), 20 days (n=4), and 30 days (n=3) post-injection. Results were normalized to uninjected birds sacrificed at ~60 days of age (n=3). Area X FoxP2 levels (blue bars), were significantly elevated at 20 days post-injection and remained so at 30 days post-injection. No such increases were observed in outlying VSP (yellow bars).