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To confirm our ChIP-PCR result, negative controls β actin (-500 bp upstream of TSS) and Cntnap2 (-2500 bp upstream of TSS of Cntnap2) show no enrichment of FoxP2 pulled down DNA by qPCR. Alternatively, primers amplifying the proposed FoxP2 binding motif and the adjacent regions, show increased signal, confirming our ChIP-PCR result.
