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. 2018 Feb 20;9:257. doi: 10.3389/fmicb.2018.00257

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Copyright © 2018 Hao, Cui and Qu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Diagram of CRISPR/Cas9 system-mediated gene editing process. pCas9 plasmid consists of cas9 gene, sgRNA, resistance locus and homologous arms. Cas9 and sgRNA gene express in order to identify target DNA and cleave at specific sites in front of PAM. RuvC and HNH are both nuclease domain in Cas9, and they cleave target DNA strand and its antisense strand, respectively. Homologous arm sequences in plasmid are complementary to their counterparts in genome so that the impact target gene fragment can be selected accurately. The whole target DNA is able to be deleted, replaced or added with a piece of new sequence through design of different homologous repair arm.