Table 1.
The resistance gene(s) in CCD1 and β-lactam MICs for the New Delhi metallo-β-lactamase (NDM)-carrying Escherichia coli clinical isolate, transconjugants, and transformants.
| Antibioticsa | MIC (mg/L) for strains |
||||||
|---|---|---|---|---|---|---|---|
| CCD1 | 20J3 | J53 | DH5α+ pHSG398 | DH5α+ pHSG398/NDM-20 | DH5α+ pHSG398/NDM-5 | DH5α+ pHSG398/NDM-1 | |
| Ampicillin | >512 | >512 | 2 | 2 | 512 | 512 | 512 |
| Amoxicillin | >512 | 512 | 16 | 1 | 512 | 512 | 512 |
| Amoxicillin+CLA | 128 | 128 | 16 | 2 | 128 | 128 | 128 |
| Aztreonam | 256 | 0.12 | 0.06 | 0.06 | 0.03 | 0.03 | 0.06 |
| Ceftazidime | >512 | >512 | 1 | 0.5 | 512 | 256 | 128 |
| Cefoxitin | 512 | 512 | 8 | 4 | 256 | 128 | 256 |
| Cefazolin | >512 | >512 | 4 | 2 | 256 | 256 | 256 |
| Cefotaxime | 512 | 512 | 0.12 | 0.03 | 32 | 32 | 32 |
| Cefepime | 512 | 256 | 0.12 | 0.015 | 4 | 2 | 2 |
| Ertapenem | 128 | 64 | 0.06 | 0.015 | 1 | 2 | 0.25 |
| Imipenem | 128 | 64 | 0.5 | 0.125 | 4 | 4 | 4 |
| Meropenem | 128 | 64 | 0.12 | 0.03 | 2 | 4 | 2 |
| Penicillin G | >512 | >512 | 32 | 16 | 512 | 512 | 512 |
| Piperacillin | >512 | >512 | 16 | 0.5 | 16 | 16 | 16 |
| Piperacillin+TZB | >512 | >512 | 16 | 0.5 | 16 | 16 | 16 |
| Colistin | 0.25 | 0.12 | 0.25 | 0.12 | 0.12 | 0.12 | 0.12 |
| Gentamicin | 1 | 2 | 2 | 1 | 1 | 1 | 1 |
| Tigecycline | 0.06 | 0.06 | 0.06 | 0.06 | 0.06 | 0.03 | 0.03 |
aCLA, clavulanic acid (fixed concentration of 2 mg/L); TZB, tazobactam (fixed concentration of 4 mg/L).