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. 2018 Feb 21;9:312. doi: 10.3389/fmicb.2018.00312

Figure 3.

Figure 3

Phenotypical analyses of the CRISPR/Cas9-ΔSCO4117 mutant and SCO4117 gene expression. (A) Macroscopic view of aerial mycelium development (white color) in the ΔSCO4117 mutant (1) and in the CRISPR/Cas9-ΔSCO4117 mutant (2), both grown in SFM plates at 2 days. (B) Actinorhodin (sucrose-free R5A extracellular medium, purple color), CDA and undecylprodigiosin production in the ΔSCO4117 and CRISPR/Cas9-ΔSCO4117 mutants compared to the wild-type strain. (C) SCO4117 transcript abundance at 17, 48, and 72 h in the S. coelicolor wild-type strain.