Table 5.
Mutation density in HorTILLUS population based on 32 genes TILLed.
| Gene | No. of M2 plants analyzed | Length of amplicon (bp) | Amplicon GC content (%) | Amount of nucleotides scanned (bp) | No. of mutations detected | Mutation density (mut/kb) |
|---|---|---|---|---|---|---|
| Dhn5 | 3,072 | 768 | 65.5 | 2,359,296 | 4 | 1/590 |
| HVA1 | 3,072 | 700 | 65.1 | 2,150,400 | 16 | 1/134 |
| HvABI5 | 6,144 | 1,072 | 62.9 | 6,586,368 | 28 | 1/235 |
| HvAPY2 | 3,072 | 935 | 41.6 | 2,872,320 | 10 | 1/287 |
| HvBAK1 | 3,072 | 778 | 39.2 | 2,390,016 | 6 | 1/398 |
| HvCBP20/1 | 5,376 | 706 | 53.0 | 3,795,456 | 18 | 1/211 |
| HvCBP20/2 | 5,376 | 730 | 39.4 | 3,924,480 | 11 | 1/357 |
| HvCBP20/3 | 5,376 | 787 | 39.7 | 4,230,912 | 3 | 1/1,410 |
| HvCBP80 | 3,072 | 691 | 39.5 | 2,122,752 | 12 | 1/177 |
| HvCENH3 | 6,144 | 574 | 57.9 | 3,526,656 | 7 | 1/504 |
| HvDMC1 | 5,376 | 811 | 41.6 | 4,359,936 | 6 | 1/727 |
| HvDREB1 | 4,608 | 812 | 49.9 | 3,741,696 | 5 | 1/748 |
| HvDRF1 | 3,072 | 724 | 51.2 | 2,224,128 | 16 | 1/139 |
| HvDWARF | 3,072 | 701 | 42.8 | 2,153,472 | 13 | 1/166 |
| HvERA1 | 4,608 | 822 | 39.0 | 3,787,776 | 14 | 1/271 |
| HvEXPB1/1 | 3,072 | 705 | 57.7 | 2,165,760 | 9 | 1/241 |
| HvEXPB1/2 | 3,072 | 525 | 57.9 | 1,612,800 | 1 | 1/1,613 |
| HvGNA1 | 5,376 | 552 | 67.7 | 2,967,552 | 12 | 1/247 |
| HvHPA1 | 5,376 | 1,100 | 38.9 | 5,913,600 | 11 | 1/538 |
| HvHTD1 | 6,912 | 971 | 60.9 | 6,711,552 | 16 | 1/419 |
| HvHTD2 | 6,912 | 1,146 | 60.0 | 7,921,152 | 9 | 1/880 |
| HvHTD3 | 6,144 | 1,126 | 40.1 | 6,918,144 | 9 | 1/769 |
| HvHTD4 | 6,912 | 1,039 | 69.6 | 7,181,568 | 7 | 1/1,026 |
| HvHTD5 | 6,912 | 1,084 | 75.5 | 7,492,608 | 9 | 1/833 |
| HvHTD6 | 6,912 | 1,147 | 63.1 | 7,928,064 | 10 | 1/793 |
| HvKu70/1 | 6,144 | 1,150 | 44.7 | 7,065,600 | 1 | 1/7,066 |
| HvKu70/2 | 6,144 | 1,100 | 38.4 | 6,758,400 | 5 | 1/1,352 |
| HvKu70/3 | 6,144 | 1,190 | 41.8 | 7,311,360 | 1 | 1/7,311 |
| HvKu80/1 | 5,376 | 569 | 42.6 | 3,058,944 | 7 | 1/437 |
| HvKu80/2 | 5,376 | 651 | 42.9 | 3,499,776 | 5 | 1/700 |
| HvLSD | 3,072 | 998 | 41.5 | 3,065,856 | 6 | 1/511 |
| HvPARP3/1 | 5,376 | 527 | 44.2 | 2,833,152 | 1 | 1/2,833 |
| HvPARP3/2 | 5,376 | 828 | 45.4 | 4,451,328 | 0 | – |
| HvPARP3/3 | 5,376 | 805 | 46.3 | 4,327,680 | 10 | 1/433 |
| HvPRT6 | 6,912 | 1,083 | 41.2 | 7,485,696 | 11 | 1/681 |
| HvRAA1 | 5,376 | 545 | 58.8 | 2,929,920 | 34 | 1/86 |
| HvRTH3 | 6,912 | 1,015 | 54.7 | 7,015,680 | 5 | 1/1,403 |
| HvSNAC1 | 6,021 | 1,277 | 64.0 | 7,688,817 | 19 | 1/405 |
| HvUVRD | 6,144 | 877 | 39.5 | 5,388,288 | 9 | 1/599 |
| HvWRKY38 | 3,072 | 730 | 63.5 | 2,242,560 | 6 | 1/374 |
| Total | 182,161,521 | 382 | 1/477 |