Table 3.
Molecular identification of Algerian F. oxysporum (Fo) isolates based on patterns of SIX3 and SIX4 gene amplifications (+, amplicon was present; −, amplicon was absent).
Isolates | Primer pairs | Fo identify | ||
---|---|---|---|---|
SIX3-F1/SIX3-R2 | SIX4-F1/SIX4-R1 | SIX3-G137C-F1/SIX3-R2 | ||
(608 bp) | (967 bp) | (412 bp) | ||
F1, F3, F5, F11, F15, F16, F21, F23-F25, F27, F30, F33, F37-F39, F42, 4287a | + | − | − | Race 2 FOL |
F12, F20 | + | − | + | Race 3 FOL |
F2, F6, F8, F9, F13, F17, F22, F26, F43, F44 | ||||
Forlcb | − | − | − | FORL |
Isolate 4287 was included as reference for f. sp. lycopersici (FOL) race 2.
Isolate Forlc was included as reference for f. sp. radicis lycopersici (FORL).