Table 5.
Source and identification of Algerian Trichoderma isolates analyzed in this study.
| Isolate code | Source/geographical origin | Identified as | 100% Identity to strain/accession numbera |
|---|---|---|---|
| T1 | Soil/Tipaza | T. harzianum | GJS 99-5/FJ442236 |
| T2 | Soil/Tipaza | T. asperellum | CBS 433.97/AF456907 |
| T3 | Soil/Tipaza | T. asperellum | CBS 433.97/AF456907 |
| T4 | Soil/Tipaza | T. asperellum | CBS 433.97/AF456907 |
| T5 | ITCMIb nursery/Staouali Algiers | T. harzianum | GJS 99-5/FJ442236 |
| T6 | Soil/Corso, Boumerdes | T. harzianum | GJS 99-5/FJ442236 |
| T7 | Soil/Corso, Boumerdes | T. harzianum | GJS 99-5/FJ442236 |
| T8 | ITCMIb nursery/Staouali Algiers | T. ghanense | DAOM 220800/EU280100 |
| T9 | Soil/Jijel | T. asperellum | CBS 433.97/AF456907 |
| T10 | Soil/Jijel | T. asperellum | CBS 433.97/AF456907 |
| T11 | Soil/El Kennar, Jijel | T. asperellum | CBS 433.97/AF456907 |
| T12 | Soil/El Kennar, Jijel | T. asperellum | CBS 433.97/AF456907 |
| T13 | Soil/El Kennar, Jijel | T. asperellum | CBS 433.97/AF456907 |
| T14 | Soil/Jimar, Jijel | T. asperellum | CBS 433.97/AF456907 |
| T15 | Soil/Djimar, Jijel | T. asperellum | CBS 433.97/AF456907 |
| T16 | Soil/Djimar, Jijel | T. asperellum | CBS 433.97/AF456907 |
| T17 | Soil/Skikda | T. asperellum | CBS 433.97/AF456907 |
The classification of Trichoderma species was based on the sequences obtained from ITS1-ITS4 region of the rDNA and a fragment of tef1alpha gene.
a
100% identity of ITS and tef1alpha-fragment sequences with database (NCBI GenBank) sequences from reference Trichoderma strains.
b
ITCMI: Industrial and Vegetable Crops Institute, Staouali Algiers, Algeria. Strains are available for research proposals in the Mycological Collection of the Biotechnology Research Center (CRBt, Constatine, Algeria) (www.crbt.dz).