Figure 2.

TCS improves p62 expression and strengthens the co-localization among intracellular pathogens, LC3 and p62. RAW264.7 cells were transfected with RFP-LC3 plasmids and incubated with or without pathogens for 30 min, and then the cells were treated with or without TCS (8 μM) for 90 min, followed by immunofluorescent staining. p62 was marked by a p62 antibody, and the pathogens and nuclei were stained by Hoechst 33342. The results were collected by a laser-scanning confocal microscope (A). Scale bars = 10 μm. The cells were treated with TCS (8 μM) for different times ranging from 0 to 90 min, and the levels of p62 were assayed by western blot (B). Compared to the respective controls.