Figure 3.

The pathogen clearance ability is enhanced by TCS treatment in vitro and in vivo. RAW264.7 cells were incubated with Rapa (200 nM, 12 h), 3-MA (5 mM, 180 min), and TCS (8 μM, 90 min) (A) or TCS (0–64 μM, 90 min) (B) first and then infected with S. typhimurium at an MOI of 25:1. Colony counting was used to calculate the extracellular CFU. RAW264.7 cells were incubated with S. typhimurium at an MOI of 25:1 for 120 min. The cells were then treated with Rapa (200 nM, 12 h), 3-MA (5 mM, 180 min), and TCS (8 μM, 90 min) (C) or TCS (0–64 μM, 90 min) (D) for 90 min. Colony counting was used to calculate the intracellular CFU. Female BALB/c mice were infected with S. typhimurium for 24 h, and the mice were treated with TCS once a day for 4 days. The livers (E) and spleens (F) were homogenized for colony counting. BALB/c mice were infected with S. typhimurium for 24 h, and the mice were treated with or without 3-MA (24 mg/kg) first and were then injected with TCS once a day for 4 days. The livers and spleens were homogenized for colony counting (G,H). Compared to the respective controls, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.