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. 2018 Feb 21;9:268. doi: 10.3389/fmicb.2018.00268

Figure 4.

Figure 4

RyhB regulation of ScyA and CctA expression is mediated by sequences in the start of the genes. (A) Predicted interactions between RyhB and scyA and cctA mRNAs. Predictions were made with IntaRNA (Busch et al., 2008; Wright et al., 2014; Mann et al., 2017). Nucleotides are numbered according to their transcription start site (Shao et al., 2014). The transcription start site is located 66 nt upstream from the translational start for scyA. cctA contains two transcription start sites located 85 and 131 nt upstream from the translational start site, respectively (the numbers in the figure refer to the start site 131 nt upstream the translational start, which is used for the cctA-lacZ fusion). Nucleotides marked in red are designating the end of scyA codons in the fusion scyA-14-lacZ. (B) Regulation of target gene fusions by expression of RyhB. β-galatosidase activity was quantified from gene-lacZ fusions harbored on a plasmid in the presence of another plasmid with inducible RyhB production, pKM033-ryhB (pRyhB), or in the presence of an empty plasmid, pKM033 (p). pKM232 contains a promoter-less and truncated lacZ gene missing the first 8 codons. Data are from two independent experiments, each with duplicate cultures, and triplicate samples. Activity is expressed as the mean (Miller units) ± SD.