Fig 3. Functional annotation of the 190 proteins of the pyrenoid proteome.

Proteins were classified according to KEGG for C. reinhardtii using a three Gene Ontology annotation levels. The KEGG annotations were further refined manually to optimize functional categories. Proteins involved in CO₂ fixation, CO₂ metabolism (such as carbonic anhydrases) or annotated as `low CO<sub>2</sub> inducible`genes are included in node “Photosynthesis and CO₂ metabolism”. The node “Carbohydrate” has proteins in carbohydrate (principally starch) metabolism and proteins harboring an alpha amylase catalytic domain or a starch-binding domain. Nodes are labeled either with the gene name or with the UniprotKB identifier. The functional categories and the properties of each protein are detailed in S3 Table.