Fig 5. Immunohistological staining for α-synuclein.

Immunohistological staining for α-synuclein was performed in brain tissue from 3 infantile KD patients (A-C), a late-onset KD patient (D), 2 patients found to be GALC mutation/variant carriers with no neurological disorders previously diagnosed (E,F), a Parkinson’s patient (G), and a healthy control with no GALC mutations (H). Accumulations of α-synuclein were observed in all Krabbe patients (A-D), the Parkinson’s brain (G), and also within the brains of GALC mutation/variant carriers (E,F). Accumulations in the infantile brains were abundant but smaller in size than the Lewy bodies observed in the Parkinson’s brain and the α-synuclein accumulations found in late-onset KD and GALC mutation carrier tissue. No significant α-synuclein accumulation was observed in the healthy diagnosed patient without GALC mutations (H). A secondary control experiment confirmed the specificity of the secondary antibody (I). Scale Bars: 100 μm.