Figure 1. Morphology and Light Responses of Pix_ON-RGCs.

(A) Orthogonal projections of a two-photon image stack through a representative Pix_ON-RGC filled during physiological recording in Grik4-Cre:Ai9 mice.

(B) Length of the longest axis through a polygon around the PixON dendritic field (n = 38), equivalent diameter of the Pix_ON-RGC dendritic field (n = 38), and total dendritic length (n = 23) of Pix_ON-RGCs.

(C) Sholl analysis of traced Pix_ON-RGC dendrites (n = 23).

(D) Stratification of Pix_ON-RGC dendrites within the IPL (n = 18; 0%–100% border between inner plexiform layer and inner nuclear layer to border between inner plexiform layer and ganglion cell layer). In (C) and (D), lines (shaded areas) indicate the means (±SEMs) of the traced population.

(E) Representative spiking (black), and excitatory (red) and inhibitory (cyan) currents in response to presentation of a 300-µm circle (2 s ON, 2 s OFF; 1,500 R*/rod/s background) centered on the soma of the recorded cell. Dashed lines show baselines in the absence of stimulus.

(F) Spontaneous and peak firing rates (n = 23) in response to the same stimulus as in (E).

(G) Percentage of peak spike response remaining 1.5 s after stimulus onset (n = 23).

(H) Change in excitatory and inhibitory conductance (n = 38) in response to the same stimulus as in (E).

(I) Percentage of peak excitatory conductance remaining 1.5 s after stimulus onset (n = 38).

In (F)–(I), dots represent data of individual cells, whereas larger circles (error bars) indicate means (±SEMs) of the respective populations. See also Figures S1, S2, and S4.