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. Author manuscript; available in PMC: 2018 Feb 26.
Published in final edited form as: Cell Rep. 2018 Feb 13;22(7):1787–1797. doi: 10.1016/j.celrep.2018.01.016

Figure 6. Heterologous Production of the O4 Antigen by S. Paratyphi A Activates the Respiratory Burst in Neutrophil-like Cells through Antibody-Mediated Complement Activation.

Figure 6

(A) HL60 cells were infected with the indicated opsonized bacterial strains and ROS production in the presence (+) or absence (-) of EGTA and magnesium (Mg2+) was monitored over time using chemiluminescence.

(B) The indicated serum dilutions were applied to a dot blot to detect the presence of IgM using anti-human IgM antibody (α-IgM).

(C) HL60 cells were infected with the indicated bacterial strains opsonized with IgG-depleted serum or IgM-depleted serum and ROS production monitored over time using chemiluminescence.

(A and C) Chemiluminescence was quantified from three independent experiments at the indicated time point after infection. Bars represent means ± standard error.

Mock, mock infection; NS, not statistically significantly different; RLU, relative luminescence units; wt, wild-type. *p < 0.05; **p < 0.001.