Figure 1.

OmpA₁₇₁ populates an additional conformation to the canonical folded and unfolded states during SDS-PAGE. (A) Representative gel and corresponding densitometry values for OmpA₁₇₁ folding into diC₁₂PC LUVs. The bands corresponding to the folded and unfolded conformations on the gel are indicated by “F” and “U”, respectively. The gel has been darkened for better clarity but densitometry analysis was performed on the unaltered scan. (B) Fractions folded and unfolded for OmpA₁₇₁ folding into diC₁₂PC. Fractional quantities were calculated by dividing by the sum of the folded and unfolded band intensities (“F+U”) or by the boiled band intensity (“B”). Solid lines are fits to a quadruple exponential expression.