Figure 5.

CD wavelength spectra of OmpA₁₇₁ immediately after initiating folding into LUVs of (A) diC₉PC, or (B) diC₁₃PC (dotted lines) at 25 °C, and after incubation with diC₁₃PC at 4 °C (B; orange line). “Initial” wavelength data were collected in 10 nm sections to capture the signal at the earliest times possible, and then combined into a full spectrum. The spectrum at 4 °C (B) was collected at the conclusion of kinetics measurements at 4 °C, and scaled by a factor of 1.25 to correct for the temperature dependence of the signal (see Supporting Information). Also included in each plot for comparison are the spectrum for unfolded OmpA₁₇₁ (dashed red line), and the spectrum measured after folding was complete in the corresponding lipid at 25 °C (solid light blue and purple lines), from Figure 3B.