Figure 3. ieCPCs Acquire Transcriptional Signatures of Developing CPCs.
(A) Transcriptome analysis revealing differences in gene expression among passage 3 (P3) and passage 12 (P12) ieCPCs, their parental MEFs, cells at reprogramming D9 (D9), and ieCPC cardiac derivatives (ieCPC-CDs) detected by RNA-seq.
(B) GO analyses of upregulated and downregulated genes in ieCPCs P3/MEFs.
(C) Principal-component (PC) analysis of the global gene expression profile across all tested cell types. CPs, cardiac progenitors; D_, Devine et al. (2014); MES, mesoderm; Pos and Tot, CPCs with or without purification with a Smarcd3-GFP+ reporter, respectively; W_, Wamstad et al. (2012).
(D) Expression of CPC-related marker genes in all tested samples detected by RNA-seq.
See also Figure S4.