FIG. 5.

2DFS rephasing spectra of the Cy3 monomer [(a)–(d)] and the (Cy3)₂ dimer-labeled DNA constructs [(e)–(h)]. [(a) and (e)] Spectral overlap of the absorbance and laser excitation. [(b) and (f)] 2DFS rephasing spectra of the monomer and dimer constructs. The concentric circles indicate the laser spectral overlap. The diagonal and anti-diagonal 2D cross sections are marked with dashed lines. [(c) and (g)] Anti-diagonal lineshape of the monomer and dimer fit to Lorentzian and Gaussian functions, respectively. [(d) and (h)] Diagonal lineshape of the monomer and dimer fit to Lorentzian and Gaussian functions, respectively.