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. Author manuscript; available in PMC: 2018 Dec 4.
Published in final edited form as: Mol Pharm. 2017 Nov 15;14(12):4685–4693. doi: 10.1021/acs.molpharmaceut.7b00775

Figure 3.

Figure 3

Determination of hPepT1-mediated substrate transport in Xenopus oocyte expression system. (A–C) Currents induced by compounds 3, 7, 10, and 39, glycylsarcosine and aspartame were monitored in hPepT1 cRNA-injected oocytes at pH6.0 using TEVC technique with varying membrane potential. (D and E) Inward currents induced by compounds 3, 7, 10, and 39, aspartame and 4-amino phenyl acetic acid (4-amino PA) were monitored at −50 mV and were expressed as 100% of 1 mM or 250 µM glycylsarcosine induced currents. (F) Tracers of representative 250 µM glycylsarcosine, 30 mM aspartame, and 10 mM 4-amino PA induced currents in hPepT1 cRNA-injected oocytes at pH6.0. Induced currents were monitored at membrane potential clamped at −50 mV.