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. 2018 Feb 6;70(3):371–382. doi: 10.1002/art.40386

Figure 2.

Figure 2

Inactivation of SOXC genes in the Prg4 + cell lineage does not affect joint integrity in young mice. A, Schematic representation of the timeline of the experiment. Tamoxifen (tmx) was injected on postnatal days 21 (P21) and 60. B, RNA in situ hybridization in adjacent mouse knee sections, using Sox4 and Sox11 RNA probes. Expression of Sox4 and Sox11 was down‐regulated in synovium and articular chondrocytes from Sox4 fl/fl 11 fl/fl 12 –/– Prg4 CreERt2 mouse knees. C, Fold changes in Sox4 and Sox11 mRNA levels in pooled knee and hip synovial tissue samples (n = 3 mice per genotype), as assessed by quantitative reverse transcription–polymerase chain reaction. Values are the mean ± SD. ** = P < 0.01. D and E, Safranin O–stained knee synovium (D), and fast green–counterstained tibial cartilage (E). F = femur (see Figure 1 for other definitions). Original magnification × 20 in B, D, and E.