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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Cell Biochem. 2018 Jan 9;119(4):3697–3705. doi: 10.1002/jcb.26586

Fig. 2. Increase of expression of neuroendocrine phenotypic marker by epidrug (5-Aza) in PCa cells.

Fig. 2

PCa cells (PC3 or DU145) (2 × 105) were seeded in 10cm culture dishes. The cells were treated by vehicle or 5-Aza (5µM) for 10 days. (A–C) mRNA levels of N-Myc downstream regulated 1 (NDRG1), Enolase-2 (ENO2), and Synaptophysin expression in vehicle or 5-Aza (5µM) treated PCa cells (PC3 or DU145) as quantified by real-time PCR. The data in Fig. 2A–C are representative of mean ± s.d (n=6). P values were calculated by Student’s t-test. (D) Protein levels of NDRG1, ENO2, and Synaptophysin following vehicle or 5-Aza (5µM) treatments in PCa cells (PC3 or DU145) as quantified by Western blots.