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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Cell Biochem. 2017 Dec 26;119(4):3267–3279. doi: 10.1002/jcb.26486

Figure 1. Knockdown of Spry1 attenuates entry into S-phase of hAoSMC in response to growth medium stimulation.

Figure 1

Time course analysis of cell cycle progression using propidium iodide staining followed by flow cytometry. A) Representative cell cycle distribution histograms show a decrease in the fraction of S1kd hAoSMC in S-phase, and an increase of debris in these cells compared to NT control hAoSMC at 12 hours post-stimulation. B) Quantification of all phases of the cell cycle from triplicate experiments at 12 hours post-stimulation. C) Representative cell cycle distribution histograms shown for S1kd hAoSMC compared to NT control at 24 hours post-stimulation. D) Quantification of all phases of cell cycle from a triplicate experiments at 24 hours post-stimulation. E) Representative cell cycle distribution histograms of S1kd hAoSMC compared to NT control at 36 hours post-stimulation. F) Quantification of all phases of cell cycle from a triplicate experiments at 36 h post-stimulation.