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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Cell Biochem. 2017 Dec 26;119(4):3267–3279. doi: 10.1002/jcb.26486

Figure 3. Mitogenic stimulation down-regulates Spry1 expression via proteasome degradation in hAoSMC.

Figure 3

A) Immunoblotting shows that growth medium stimulation results in a rapid down-regulation of Spry1 with nadir at 6 h post-stimulation, in contrast to the continuous degradation of p27Kip1 in hAoSMC. B) Quantification of bands from A to show the difference in protein levels of p27Kip1 and Spry1 in response to mitogenic stimulation. C) Immunoblotting shows that the proteasome inhibitor MG132 alone slightly down-regulates Spry1, however it does rescued the expression of p27Kip1 and Spry1 after SmGM-2 stimulation. D) Quantification of Spry1 in panel C to show the changes in Spry1 levels in response to SmGM-2 stimulation and MG132 treatment.