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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Cell Biochem. 2017 Dec 26;119(4):3267–3279. doi: 10.1002/jcb.26486

Figure 5. Spry1 is constitutively expressed in normal arterial VSMC and transiently down regulated by injury.

Figure 5

A) X-gal staining of vascular tissues from Spry1lacZ mice, that have LacZ reporter under the control of Spry1 promoter shows staining is predominantly in VSMC and adventia but not in endothelial cells (arrows). B) Immunoblotting of the aorta lysates from Spry1+/+ or Spry1−/− mice confirms the expression of Spry1 protein Spry1+/+ vessels and Spry1−/− vessels. C) RT-qPCR analysis of a time course LCL experiment to show injury-mediated changes in Spry1, Spry4 and MHY11 gene expression. D) Immunohistochemistry (IHC) on a time course LCL experiment to show changes in expression of Spry1, Spry2, Spry4 and SMTN-B proteins.