Table 9.
Analytical characteristics of the method validation
| S. no | Parameters | Ursolic acid (UA) | Oleanolic acid | Betulinic acid |
|---|---|---|---|---|
| 1 | Linearity range (ng/spot; n = 12a) | 200–3000 | 200–3000 | 200–3000 |
| 2 | Correlation coefficient (r2) | 0.991 | 0.997 | 0.982 |
| 3 | Regression equation | Y = − 126.87.0 + 11.05X | Y = − 1111.13 + 10.0914X | Y = − 1003.11 + 6.0766X |
| 4 | Calculated SD value (CATS software) | 2.59 | 3.26 | 6.72 |
| 5 | bLimit of detection (LOD) (ng) [3 × SD/S] | 30 | 27 | 25 |
| 6 | bLimit of quantitation (LOQ) (ng) [10 × SD/S] | 90 | 81 | 75 |
| 7 | R f | 0.58 | 0.8 | 0.62 |
| Precision and accuracy | ||||
| 8 | Intra-day RSD (%), n = 5 | 0.357 | 0.487 | 0.317 |
| 9 | Inter-day RSD (%), n = 5 (day-1/day-2/day-3) | 0.353/0.425/0.532 | 0.484/0.498/0.514 | 0.315/0.376/0.412 |
| Recovery | ||||
| 10 | Amount of standard in stem samples (μg mg−1) containing highest triterpenoid | 8.1 | 19.0 | 7.0 |
| 11 | Amount of standard added in stem sample (μg mg−1) | 2/4/6 | 2/4/6 | 2/4/6 |
| 12 | Amount of standard found (μg) | 10.1/12.05/14.11 | 20.89/22.97/24.89 | 8.97/10.96/12.97 |
| 13 | Recovery (%) | 100/99.59/99.92 | 99.48/99.87/99.56 | 99.66/99.64/99.24 |
aFour concentration levels in triplicate
bSD is the standard deviation of the blank response and S is the slope of the calibration plot