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. 2018 Feb 26;8:3590. doi: 10.1038/s41598-018-22041-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Brush border abundance of Napi2a and renal expression of α-Klotho and FGFR1. (a) Original western blot of Napi2a and beta actin in BBM isolated from NCC WT and KO kidneys. Quantification of Napi2a expression in WT and KO kidneys. (b) Original western blot of α-Klotho, FGFR1 and beta actin in total kidney lysate from NCC WT and KO kidneys. Quantification of α-Klotho and FGFR1 expression in WT and KO kidneys.