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. 2018 Feb 26;8:3598. doi: 10.1038/s41598-018-22076-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological changes of activated macrophages. Immunostaining for LPS (A) and TLR4 (B) in carotid plaque revealed that in the area where the immunoreactivity for antibodies against LPS from Escherichia Coli (EC) (A) or for antibodies against TLR4 (B) was higher, macrophages CD68 positive showed signs of activation (red star). They appeared bigger compared to non-activated macrophages (arrow), which, conversely, were detected in the area with a less reactivity to LPS and TLR4 and disclosed typical round shape.