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. 2018 Feb 22;9:227. doi: 10.3389/fpls.2018.00227

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Copyright © 2018 Chen, Meng, Gu, Li, Yuan, Duan, Yang, Li and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro detection of ubiquitinated PperS-RNases. Each lane was loaded with 10 μg protein. The ubiquitination of different PperS-RNases was analyzed with the presence of PperSSK1, PperCUL1, PperSLFL1-3, and Ub. 10 μg of PperSLFL1-3 proteins was added to each lane, respectively. The lanes without His-PperS-RNase were used as negative control. For detection of MBP-PperSLFL proteins and GST fusion proteins by immunoblot, mouse anti-MBP and anti-GST monoclonal antibody (Bio-Rad) were used as primary antibodies.