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. 2018 Feb 22;9:338. doi: 10.3389/fmicb.2018.00338

FIGURE 4.

FIGURE 4

Optimal conditions for gmrA expression. (A) qRT-PCR for gmrA in exponential-phased and stationary-phased E. coli O157:H7 grown in LB or DMEM medium. qRT-PCR for gmrA in exponential-phased E. coli O157:H7 grown in LB or DMEM medium at different temperatures (B), pH (C), NaCl concentrations (D), and KCl concentrations (E). (F) qRT-PCR of gmrA in E. coli O157:H7 incubated in DMEM alone or co-incubated with Caco-2 or HeLa epithelial cells for 1 to 6 h. Data are mean ± SD, n = 3. ∗P ≤ 0.05; ∗∗P ≤ 0.01; ∗∗∗P ≤ 0.001 by Student’s t-test.