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. 2018 Feb 26;8:3652. doi: 10.1038/s41598-018-22069-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental workflow for global proteomic analysis of lysine-crotonylated proteins. (a) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and crotonyl-lysine immunoblot. Lines represent 72 and 120 hpf, respectively. (b) Schematic representation of the sequential steps used for global profiling of lysine crotonylation in zebrafish larvae. (c) Overlap of crotonylated sites and proteins in immunoprecipitation experiments performed in triplicate.