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. 2018 Feb 26;9:816. doi: 10.1038/s41467-018-03105-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Cardiac amphiregulin protein levels in myocardial tissues from healthy controls or patients with ischemic heart disease (IHD). a Amphiregulin (AREG) protein levels were determined by immunoblot after total protein isolation from control, healthy myocardial tissue (labeled C), or samples from patients with ischemic heart disease (labeled IHD). β-Actin (ACTb) served as a loading control. b Quantification by densitometry of the AREG immunoblot results relative to ACTb (n = 10 in both groups; data compared by two-sided Mann–Whitney test)