Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 26;9:816. doi: 10.1038/s41467-018-03105-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — Treatment of murine myocardial ischemia and reperfusion injury with recombinant amphiregulin. a–c Wild-type were exposed to 60 min of myocardial ischemia, followed by 120 min of reperfusion; infarct sizes were measured by double staining with Evan’s blue and triphenyltetrazolium chloride and serum samples were collected. All Infarct sizes are presented as the percentage of infarcted tissue in relation to the area-at-risk. Serum troponin levels were determined by ELISA. a Infarct sizes of C57/BL6 mice after 60 min of ischemia and 120 min reperfusion, treated with vehicle or 10 µg recombinant murine AREG, administered over a 15 min period via an indwelling arterial catheter. Data presented are the percentage of infarcted area in relation to area-at-risk. Statistical significance assessed by two-sided, unpaired Student’s t-test (n = 7 per group; data presented as mean ± SD). b Representative infarct staining of C57/BL6 treated with vehicle or 10 µg recombinant murine AREG. c Serum troponin levels of C57/BL6 mice, after 60 min ischemia, 120 min reperfusion, treated with vehicle or 10 µg recombinant murine Areg. Statistical significance assessed by two-sided, unpaired Student’s t-test (n = 5 per group; data presented as mean ± SD). d Areg^+/+ or Areg^−/− mice received treatment with vehicle or 10 μg of recombinant murine AREG, administered over a 15 min period by an indwelling catheter. The animals underwent 60 min ischemia and 120 min reperfusion, followed by total protein isolation form the area-at-risk. Upper panel: protein was immunoblotted for AREG, β-actin (ACTb) served as a loading control. Lower panel: protein was immunoblotted for total AKT (tAKT) and phosphorylated AKT (pAKT), respectively. Two samples for each condition and genotype are presented. One representative blot out of three experiments is shown. e, f Quantification by densitometry of the AREG and pAKT immunoblot results relative to ACTb or Total AKT (tAKT). Data are expressed as mean fold change ± SD normalized to vehicle treated Areg^+/+ and compared by one-way ANOVA followed by Bonferroni’s multiple comparison test (Areg^+/++Vehicle n = 5; all other groups n = 6 mice per group, e F_3,19 = 23,11, p < 0.0001; f F_3,20 = 9,549, p = 0.0002)