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. 2018 Feb 22;9:226. doi: 10.3389/fpls.2018.00226

FIGURE 2.

FIGURE 2

Generation of GsSRK-f and GsSRK-t transgenic alfalfa. (A) Structures showing the conserved domains of GsSRK-f and GsSRK-t. The arrows labeled with FP and RP indicated the positions of the primers used for semi-quantitative RT-PCR analysis. (B) Structures showing the constructs for GsSRK-f and GsSRK-t overexpression in alfalfa. Construct for GsSRK-f overexpression contains the full-length GsSRK gene, while construct for GsSRK-t overexpression contains a truncated version of GsSRK-f, without the N-terminal signal peptide and G-type lectin domain. (C) Semi-quantitative RT-PCR identification of GsSRK-f transgenic lines. (D) Semi-quantitative RT-PCR identification of GsSRK-t transgenic lines. The GAPDH gene in alfalfa (Accession No.: Medtr3g085850) was used as an internal control.